Fast-acting and nearly gratuitous induction of gene expression and protein depletion inSaccharomyces cerevisiae
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چکیده
منابع مشابه
Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae
We describe the development and characterization of a system that allows the rapid and specific induction of individual genes in the yeast Saccharomyces cerevisiae without changes in nutrients or temperature. The system is based on the chimeric transcriptional activator Gal4dbd.ER.VP16 (GEV). Upon addition of the hormone β-estradiol, cytoplasmic GEV localizes to the nucleus and binds to promote...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 2011
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.e11-05-0466